16S rRNA Sequencing: Discover the Advantages of Long-Read Sequencing

CeGaT is expanding its Research and Pharma Solutions product portfolio and now offers Full-Length 16S Sequencing. The new service enables full-length sequencing of the 16S rRNA gene, including all variable regions. PacBio’s Single Molecule Real-Time (SMRT) technology generates HiFi reads with exceptional accuracy and length. Thus, microbial taxa can be accurately determined.

16S rRNA sequencing is one of the most widely used methods to determine taxonomic distribution in bacterial communities, such as the gut microbiome. The highly conserved 16S rRNA gene is approximately 1.500 bp long and consists of nine hypervariable regions (V1 – V9) that differ between different bacteria.

Using PacBio’s Sequel IIe system, the entire 16s rRNA gene is sequenced. This technology enables precise long-read sequencing (HiFi reads) generated by circular consensus sequencing. As a result, the full-length gene can now be analyzed at CeGaT.

CeGaT’s Full-Length 16S Sequencing is featured by the following:

  • fast turnaround times and the highest sequencing accuracy
  • analysis of the complete 16S rRNA gene (V1-V9 regions)
  • HiFi long reads of approx. 1,500 bp
  • simultaneous analysis of multiple samples

Please use the potential of precise Full-Length 16S Sequencing and benefit from our many years of experience in NGS and diagnostics and our accredited laboratory procedures. We gladly advise you on your study or research project.

For more information, please get in touch with us at rps@cegat.com.

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