Transcriptome sequencing is a very sensitive method to analyze coding- and non-coding RNAs of an organism, tissue, or cell culture. To guide our customer in finding the best solution for his research project or clinical study, we released our brand-new Tech Note. This Tech Note visualizes our workflow, which has been specifically designed to meet our customer’s needs.
The transcriptome comprises all RNAs, which are transcribed in an organism or cell at a certain point in time. It may be influenced by the metabolic state or by external factors, such as drugs or diseases. Depending on the experimental setup or research objective, total RNA or messenger RNA (mRNA) can be sequenced. Besides common species such as humans, rats, or mice, we can also sequence the transcriptome of plants, fungi, bacteria, viruses, insects, fish, and many more. We accept either purified RNA, tissue, or cells. Our latest Tech Note guides you through the CeGaTs Transcriptome portfolio to help you find the best solution for your project.
Coding transcriptome sequencing (CTS) to capture influences on gene expression
Only about 1-5% of the transcriptome corresponds to messenger RNA. This requires high-quality RNA from cells, plants, and fresh or frozen tissues. Polyadenylation at the 3′ end of mRNA is found in most eukaryotes and is used to separate mRNA from total RNA. This mRNA enrichment results in increased sequencing depth. Even lowly expressed or rare transcripts can be detected. Coding transcriptome sequencing is commonly used to determine gene expression and for differential gene expression analysis or the characterization of alternative splicing patterns.
Whole transcriptome sequencing (WTS) for the identification and characterization of known or novel transcripts all over the transcriptome
The entity of coding RNA plus a variety of different non-coding RNAs is comprised of total RNA. Since ribosomal RNA comprises most of the total RNA, we recommend rRNA depletion before we start with the sequencing library preparation. To achieve the best possible results, CeGaT offers different kits depending on the different sample materials. Total RNA can be obtained from blood, cells, tissue as well as fresh frozen tissue. In addition, CeGaT offers special kits that can handle FFPE-embedded tissue. The analysis of total RNA enables a genome-wide comprehensive overview of the transcriptome of an organism or cell to investigate introns, exons, or splicing mechanisms.
CeGaT´s transcriptome sequencing service offers reliable and accurate insights into RNA from extraction to data analysis. With our long-standing expertise in the diagnostic field, our clients benefit from strict quality control standards and therefore high-quality results.
All sequencing data are stored in-house and are processed in-house by our own bioinformatics unit. We offer sophisticated solutions for your needs and different options for data transfer. Our detailed project report is delivered with every sequencing order by your personal contact person at CeGaT.
For further information, please do not hesitate to contact us at firstname.lastname@example.org.Transcriptome Sequencing – Tech Note