STUDYING MICROBIAL COMMUNITIES USING LONG-READ SEQUENCING
The 16S ribosomal RNA (rRNA) gene is approximately 1.5 kb long and contains several conserved and hypervariable regions (V1-V9) that vary between different bacteria. These hypervariable sequences can be used to identify and characterize microbial diversity. Therefore, the 16S rRNA gene is a common marker to characterize microbial communities in various specimens.
Using the PacBio single molecule real-time (SMRT) technology, we accurately sequence the full-length 16S rRNA gene, covering all variable regions with an average HiFi read length of about 1.5 kb. The extraordinary accuracy and length of PacBio HiFi reads that is generated by using circular consensus sequencing (CCS) mode allows microbial taxa detection at high resolution.
Advantages of Full-Length 16S Sequencing:
- Characterized by fast speed and high precision
- Analysis of the complete 16S rRNA gene (V1-V9 regions)
- HiFi read length of about 1.5 kb
- Analysis of multiple samples in a single sequencing process
Applications of Full-Length 16S Sequencing are diverse and include:
- Characterization of different microbial communities
- Microbial biomarker detection
- Disease monitoring
- Drug development
Do you have any questions about the Full-Length 16S Sequencing service? Then, contact us directly and make an appointment with your personal project consultant today.
Turnaround time
Sequencing raw data are delivered within 15 – 20 working days.
The turnaround time (TAT) depends on provided starting material and required bioinformatics level.
Delivery
Your sequencing data will be available for download from our server.
Upon request, as well as for large sample batches, data can also be delivered via external hard drive, or uploaded to your own server.
Storage
The original samples and, as the case may be, the isolated nucleic acid will be stored at CeGaT for 3 months after delivery of the data.
Your data will be stored for 5 months on our system after data delivery.
Explore our Full-length 16S Sequencing product
Full-Length 16S Sequencing
Target regions
Complete 16S rRNA gene (V1-V9 regions)
Sample types
Isolated DNA & various other sample types on request
Sequencing technology
PacBio Sequel IIe
Read length
Approx. 1.5 kb
Output
15,000 HiFi Reads per sample
Deliverables
Project report & FASTQ files
Bioinformatics
Raw sequencing data are automatically processed. We offer different levels of bioinformatic analysis:
Level 1: Demultiplexing of the sequencing data (HiFi reads, FASTQ format)
Level 2: Taxonomic classification down to species level (if possible):
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- Generation of taxonomic tables including sequence counts and relative abundances (XLSX, TSV, and BIOM format)
- Generation of barplots showing relative abundances (PNG format)
The default level is Level 1. With increasing bioinformatic level, more data are delivered. The higher level includes the data from the lower level. In addition to the data, and independent of the analysis level, a project report is generated.
Technical Information
At CeGaT, sequencing is performed using state-of-the-art PacBios Sequel IIe System.
We are pleased to offer you our specifically designed stool sampling kit which provides convenient and unbiased self-collection of fecal material combined with our DNA isolation service. Please also reach out to us to discuss sampling and DNA extraction of various starting material.
Contact Us
Start Your Project Now
We are happy to discuss sequencing options and to find a solution specifically tailored to your clinical study or research project.
When getting in contact, please specify sample information including starting material, number of samples, preferred library prep option, preferred sequencing depth and required bioinformatics, if possible.
Further Reading
