Highly Accurate detection of Bacteria and Archaea
The development of next-generation sequencing (NGS) has significantly improved our abilities to analyze microbial communities. NGS-based techniques such as 16S sequencing have enabled scientists worldwide to understand better complex microbial communities in the human body or the environment and have largely replaced classical culture-dependent techniques.
Analyzing 16S sequencing data allows bacterial and archaeal profiling down to the genus level. This has been shown using microbial community standards. The method is based on amplifying and sequencing the 16S ribosomal RNA (rRNA) gene, a common marker for bacteria and archaea. As a major advantage over other techniques, it allows the analysis of bacteria and archaea in samples with high proportions of host DNA (e.g., from plants or humans). This makes 16S sequencing the method of choice for samples with relatively low numbers of bacteria and archaea, such as isolated DNA from the skin, salvia, plant material, tumor tissue, or body fluids.
Applications of 16S sequencing are diverse and include:
- Disease monitoring
- Microbial biomarker detection
- Drug development
- Characterization of bacterial and archaeal communities
Turnaround time
The turnaround time (TAT) highly depends on the batch size, the starting material, and the requested bioinformatics level.
Delivery
Your sequencing data will be available for download from one of our servers (FTAPI or SFTP). Upon request, as well as for large sample batches, data can also be delivered via external hard drive or uploaded to your own server.
Storage
The original samples and, if applicable, the isolated nucleic acids will be stored at CeGaT for a period of 3 months after data delivery.
Data will be irrevocably deleted after 5 months.
Explore our 16S Sequencing product portfolio
16S Sequencing
Target regions
V3-V4 regions of the 16S rRNA gene
Sample types
Isolated DNA & various other sample types on request
Sequencing technology
Illumina
Read length
2 × 250 bp
Output
25,000 clusters (12,5Mb)
Deliverables
Project report & FASTQ files
Please note that a minimum of 60 samples per batch is required.
Bioinformatics
Raw sequencing data are automatically processed in order to facilitate immediate genetic evaluation.
Included in delivery are demultiplexed, and adapter trimmed FASTQ files.
Further options for bioinformatic analyses are available:
- Mapping against 16S database (RMA format) *
- Taxonomic classification using MEGAN6 (MEGAN format) *
- Generation of taxonomic tables including sequence counts and relative abundances down to the genus level (XLS and BIOM format)
- Generation of barplots showing relative abundances down to the genus level (PNG format)
- Generation of a detailed project report (PDF format)
* These files can be interactively explored and further analyzed using MEGAN6, a user-friendly and open source software for analyzing microbiome data.
Technical Information
At CeGaT, paired-end sequencing (2 x 250 bp) is performed using state-of-the-art Illumina MiSeq Sequencing Systems.
If you require sequencing parameters other than those presented in our product portfolio, please let us know! We can provide further solutions.
We are pleased to offer you our specifically designed stool sampling kit which provides convenient and unbiased self-collection of fecal material combined with our DNA isolation service. Please also reach out to us to discuss sampling and DNA extraction of various starting material.
Contact Us
Start Your Project Now
We are happy to discuss sequencing options and to find a solution specifically tailored to your clinical study or research project.
When getting in contact, please specify sample information including starting material, number of samples, preferred library prep option, preferred sequencing depth and required bioinformatics, if possible.
Further Reading
