Receive the most comprehensive collection of an individual`s genetic information
The genome represents the entire genetic information of an organism. Analyzing the whole genome using next-generation sequencing (NGS) technology provides the most comprehensive collection of an individual’s genetic information. By comparing the individual to a reference genome, variations can be studied. These alterations can include single-nucleotide variants (SNVs), small insertions and deletions (indels), copy-number variations (CNVs), and structural variants (SVs).
Whole genome sequencing (WGS) studies contribute to:
- Cancer studies, personalized medicine approaches, translational research
- Discovery of biomarkers and understanding pharmacogenetics
- Disease research
- Plant and animal breeding programs
- Examination of microorganisms
Turnaround time
Sequencing data are delivered within 15 – 20 business days.
A fast track option is available upon request for larger batch sizes.
The turnaround time (TAT) depends on the quality of the provided DNA, and the requested bioinformatics level.
Delivery
Your sequencing data will be available for download from our server.
Upon request, as well as for large sample batches, data can also be delivered via external hard drive, or upload to your own server.
Storage
The original samples, and if applicable, the isolated nucleic acids will be stored at CeGaT for 3 months after data delivery.
Your data will be stored for 5 months.
Explore our whole genome sequencing product portfolio
WGS Large Classic
Species
Human, murine
DNA quality
High molecular weight DNA
Protocol
+/– PCR amplification
Sequencing technology
Illumina
Output
90 Gb
Deliverables
Project report & FASTQ files
WGS Small Classic
Species
Microorganisms (genome size less than 20 Mb)
DNA quality
High molecular weight DNA
Protocol
+ PCR amplification
Sequencing technology
Illumina
Output
2 Gb
Deliverables
Project report & FASTQ files
WGS Flex
Species
Diverse (genome size greater than 20 Mb)
DNA quality
Various quality (e.g. fragmented DNA)
Protocol
+/– PCR amplification
Sequencing technology
Illumina
Output
Flexible
Deliverables
Project report & FASTQ files
Bioinformatics
Raw sequencing data are automatically processed in order to facilitate immediate genetic evaluation. Included in delivery are demultiplexed and adapter trimmed FASTQ files. Further bioinformatic options are available:
Analysis of DNA samples with Illumina DRAGEN Bio-IT platform:
- Mapped data (BAM file), coverage distribution (BEDGRAPH file) and plot of the coverage distribution (PDF file)
- Single nucleotide variants (SNVs) and small insertion and deletion (indel) calling without annotation
- Detection of structural variant (SVs), such as translocations, inversions as well as large and medium-sized indels without annotation
- Copy number variation (CNV) calling
- Prediction of ploidy of sex chromosomes for human samples (CSV file)
Technical Information
At CeGaT, paired-end sequencing is performed on state-of-the-art Illumina NovaSeq 6000 Sequencing Systems.
WGS samples are sequenced in 2 x 150 bp or 2 x 100 bp mode.
If you require sequencing parameters other than those presented in our product portfolio, please let us know! We can provide further solutions.
Contact Us
Start Your Project Now
We are happy to discuss sequencing options and to find a solution specifically tailored to your clinical study or research project.
When getting in contact, please specify sample information including starting material, number of samples, preferred library prep option, preferred sequencing depth and required bioinformatics, if possible.
Further Reading
