Identify Individual HLA Alleles with Next-Generation Sequencing
The Human Leukocyte Antigen (HLA) system is a group of genes which have an important function in the human immune system. The HLA region is highly polymorphic and contains six classical HLA genes: class I HLA-A, -B and –C, and class II HLA-DRB1, -DQB1 and –DPB1. Furthermore, the HLA region is known to be associated with more than 100 multifactorial, complex diseases mainly of inflammatory and autoimmune pathogenesis. In order to find out more about the functions and complex interactions of the HLA system, next-generation sequencing (NGS) based HLA typing can be very helpful.
NGS can for example support characterisation of individual response to drug therapy including pharmacogenetics, HLA-matching of donor-recipient pairs for transplantation, or deliver important information for immunotherapy to fight cancer. A huge advantage of NGS in HLA research is the possibility of fast and precise identification of HLA alleles.
Turnaround time
Sequencing data are delivered within 15 – 20 working days.
A fast track option is available upon request.
Delivery
Your sequencing data will be available for download from one of our servers.
Upon request, as well as for large sample batches, data can also be delivered via external hard drive, or uploaded to your own server.
Storage
The original samples and, as the case may be, the isolated nucleic acid will be stored at CeGaT for 3 months after delivery of the data.
Your data will be stored for 5 months on our system after data delivery.
Explore Our HLA Typing Product Portfolio
HLA Classic
HLA type
HLA class I (HLA-A, -B and –C) and HLA class II (HLA-DRB1, -DQB1 and -DPB1)
Species
Human
DNA quality
High molecular weight DNA
HLA typing based on
Whole Exome Sequencing
Sequencing technology
NovaSeq 6000
Output
6 Gb
Deliverables
Project report & FASTQ, TSV
HLA Flex
HLA type
HLA class I (HLA-A, -B and –C) and HLA class II (HLA-DRB1, -DQB1 and -DPB1)
Species
Human
DNA quality
Flexible
HLA typing based on
Whole Exome Sequencing
Sequencing technology
NovaSeq 6000
Output
Flexible
Deliverables
Project report & FASTQ, TSV
All products can be combined with our DNA isolation service.
Bioinformatics
For HLA class I and II typing based on WES, a special algorithm is used for data evaluation. You will receive the HLA typing results as TSV file.
Thus, when we generate HLA typing data for you, we always also provide you exome data as demultiplexed and adapter trimmed FASTQ files because our HLA typing is based on exome sequencing results.
Additional to HLA typing, we can offer further bioinformatic analysis for the exome data, of course. Please find here our bioinformatic options for additional exome data analysis.
Technical Information
HLA typing results are generated out of exome sequencing data with a special bioinformatic algorithm. The following loci will be analyzed: HLA class I (HLA-A, -B and –C) and HLA class II (HLA-DRB1, -DQB1 and -DPB1)
At CeGaT exomes are sequenced paired-end (2 x 100 bp) using state-of-the-art Illumina NovaSeq 6000 Sequencing Systems.
Contact Us
Start Your Project Now
We are happy to discuss sequencing options and to find a solution specifically tailored to your clinical study or research project.
When getting in contact, please specify sample information including starting material, number of samples, preferred library prep option, preferred sequencing depth and required bioinformatics, if possible.
Further Reading
