Information
CeGaT offers sequencing of Illumina compatible Ready to Load next-generation sequencing (NGS) libraries. We make use of the latest NGS technology. Depending on the required output, samples will be sequenced on the MiSeq or NovaSeq 6000 Illumina platform.
Flexible sequencing options are available, specifically tailored for your project. Please refer to our technical information for further details.
Choosing CeGaT for your project, you will benefit from our broad experience in sequencing as well as our high quality standards, based on our German DAkkS ISO 15189 and American CAP/CLIA accreditation.
Turnaround time
Sequencing data are delivered within 5 – 10 working days.
A fast track option is available upon request.
Delivery
Your sequencing data will be available for download from one of our servers.
Upon request, as well as for large sample batches, data can also be delivered via external hard drive, or uploaded to your own server.
Storage
The original samples will be stored at CeGaT for a period of 3 months after data delivery.
Data will be stored for 5 months on our system after data delivery.
NovaSeq 6000 platform (Illumina)
SP flow cell
SR/PE
100 cycles
65 Gb
650 M Single reads CPF*
SR/PE
200 cycles
134 Gb
650 M Single reads CPF*
SR/PE
300 cycles
200 Gb
650 M Single reads CPF*
PE
500 cycles
325 Gb
650 M Single reads CPF*
S1 flow cell
SR/PE
100 cycles
134 Gb
1330 M Single reads CPF*
SR/PE
200 cycles
266 Gb
1330 M Single reads CPF*
SR/PE
300 cycles
400 Gb
1330 M Single reads CPF*
S2 flow cell
SR/PE
100 cycles
333 Gb
3335 M Single reads CPF*
SR/PE
200 cycles
667 Gb
3335 M Single reads CPF*
SR/PE
300 cycles
1000 Gb
3335 M Single reads CPF*
S4 flow cell
SR/PE
200 cycles
1600 Gb
8000 M Single reads CPF*
SR/PE
300 cycles
2400 Gb
8000 M Single reads CPF*
SR: Single-Read, PE: Paired-End, CPF: clusters passing filter.
*In case of PE sequencing, they will be twice as much.
MiSeq platform (Illumina)
Nano flow cell
SR/PE
300 cycles
0.3 Gb
1 M Single reads CPF*
PE
500 cycles
0.5 Gb
1 M Single reads CPF*
Micro flow cell
SR/PE
300 cycles
1.2 Gb
4 M Single reads CPF*
V2 flow cell
SR/PE
50 cycles
0.75 Gb
12 M Single reads CPF*
SR/PE
300 cycles
4.5 Gb
12 M Single reads CPF*
PE
500 cycles
7.5 Gb
12 M Single reads CPF*
V3 flow cell
SR/PE
150 cycles
3.3 Gb
22 M Single reads CPF*
PE
600 cycles
13.2 Gb
22 M Single reads CPF*
SR: Single-Read, PE: Paired-End, CPF: clusters passing filter.
*In case of PE sequencing, they will be twice as much.
Bioinformatics
Depending on your requirements, you will either receive raw sequencing data (BCL files) or demultiplexed FASTQ files. If needed, adapter trimming can be performed. Further bioinformatic analyses are available upon request.
Technical Information
At CeGaT, sequencing is performed using the state-of-the-art Illumina NovaSeq 6000 Sequencing System, as well as the MiSeq Sequencing System.
As starting material, we accept any Illumina-compatible library. Illumina index sequencing primers, as well as custom primers may be used during the sequencing process at CeGaT.
If you have generated libraries with commercial library construction kits for PCR-based targeted sequencing using a unique molecular identifier (UMI), we can offer sequencing modes with customized sequencing parameters. The lengths of read 1 and read 2, as well as index read lengths can be adapted to your needs.
Explore our Ready to Load sequencing product portfolio with various sequencing options for your libraries. Depending on the batch size, required output and read number specifications, you can choose from different flow cells.
Contact Us
Start Your Project Now
We are happy to discuss sequencing options and to find a solution specifically tailored to your clinical study or research project.
When getting in contact, please specify sample information including starting material, number of samples, preferred library prep option, preferred sequencing depth and required bioinformatics, if possible.
Further Reading
