Explore Cellular Diversity at Single-Cell Level
The human body consists of more than 30 trillion cells. All of them are highly heterogenous regarding their transcriptional state, even if they come from the same organ or tissue. This heterogeneity has two sides: it is an important feature of normal physiological processes, but it can also contribute to the development of cancer or immunological disorders. Traditional bulk RNA-sequencing methods are not able to resolve the heterogeneity of a cell population. But with new single-cell RNA sequencing technologies, it is now possible to analyse individual transcriptomes of thousands of cells in one sample.
CeGaT helps you to explore the cellular diversity of your samples by providing high-throughput 3‘ Single-cell RNA Sequencing (3‘ SCR). Using the state-of-the-art 10x Genomics® Chromium™ system and the latest NGS technology, we can profile 500 – 10,000 individual cells per sample in a cost-effective manner. Your project will benefit from our optimized single-cell workflow and our long-standing expertise as a sequencing provider.
Application areas of Single-cell RNA Sequencing:
- Detection of tumor heterogeneity
- Cell Differentiation & Lineage Tracing
- Response to therapeutic interventions
- Biomarker Discovery
Turnaround time
Sequencing data are delivered within 20 – 30 working days.
The turnaround time (TAT) depends on the number of samples provided.
Delivery
Your sequencing data will be available for download from our server.
Upon request, as well as for large sample batches, data can also be delivered via external hard drive, or upload to your own server.
Storage
The original samples and, if applicable, the isolated nucleic acids will be stored at CeGaT for a period of 3 months after data delivery.
Your data will be stored for 5 months on our system after data delivery.
Explore our Single-cell RNA Sequencing product portfolio
3‘ Single-cell RNA Sequencing (3‘ SCR)
Sample type
Frozen single cell suspensions
Species*
Human, murine
Sample requirements*
1 M cells in 1 ml cryopreservation medium
> 90% cell viability
Technology
Chromium™ system (10x Genomics®)
NovaSeq 6000 or MiSeq system (Illumina)
Output
Flexible
Deliverables
Project report & FASTQ files
Consulting option
Our scientists will support you with the analysis and interpretation of your single-cell data using the 10X Genomics Loupe Browser
* Other species or sample input might be possible, please contact us to discuss the options
M = million
Bioinformatics
We analyse your Single-cell RNA Sequencing data with the Cell Ranger software from 10x Genomics®. This workflow includes read alignment, generation of feature-barcode matrices, cell clustering and gene expression analysis. For each single-cell RNA sequencing project, you receive:
- Raw sequencing data (FASTQ files)
- A standard Loupe Browser visualization file (cloupe)
- Mapped count tables for downstream analysis
- A preliminary clustering analysis
- A preliminary differential gene expression analysis
- A project report, including QC values and results of the data analysis
Technical Information
We can process between 500 – 10,000 cells per sample and up to 8 samples can be run in parallel on one Chromium Next GEM Chip.
It is recommended to sequence at least 20,000 paired-end reads per cell. The required sequencing depth strongly depends on the sample type and on the experimental question being asked. At CeGaT, sequencing is performed using the state-of-the-art Illumina NovaSeq 6000 and MiSeq Sequencing Systems, allowing maximum sequencing flexibility.
Contact Us
Start Your Project Now
Our Single-cell RNA Sequencing workflow is highly flexible and can be adjusted to the number of cells in your sample and your required sequencing depth. Please contact us to discuss all the options for your clinical study or research project!
When getting in contact, please specify sample information including cell type, number of samples, number of cells per sample and preferred sequencing depth, if possible.
Further single-cell products: We are currently establishing additional 10X Genomics protocols including single cell immune profiling, single cell ATAC (Assay for Transposase Accessible Chromatin) sequencing and more!
Further Reading
