Discover small RNA molecules in your biological sample
Small RNA sequencing focuses on short RNA molecules which play an important role in silencing and post-transcriptional gene expression regulation processes. Sequencing of small RNAs allows the analysis of these molecules in more detail and therefore can be an essential part in the identification of novel biomarkers to obtain more valuable research data.
CeGaT´s small RNA sequencing service offers reliable and accurate insights into small RNAs from extraction to data analysis. With our long-standing expertise in the diagnostic field, our clients benefit from strict quality control standards and therefore high-quality results.
Turnaround time
Sequencing data are delivered within 15 – 20 working days.
A fast track option is available upon request.
The turnaround time (TAT) highly depends on the quality of the provided RNA and the requested bioinformatics level.
Delivery
Your sequencing data will be available for download from our server.
Upon request, as well as for large sample batches, data can also be delivered via external hard drive, or upload to your own server.
Storage
The original samples and if applicable, the isolated nucleic acids will be stored at CeGaT for a period of 3 months after data delivery.
Data will be stored for 5 months on our system after data delivery.
Explore our Small RNA Sequencing product portfolio
SRS Classic
Species
Human
RNA amount
> 200 ng
RNA quality
RIN ≥ 8
Sequencing technology
Illumina
Output
> 10 M clusters per sample
Deliverables
Project report & FASTQ files
SRS Premium
Species
Human
RNA amount
> 1 ng
RNA quality
Variable RNA quality
Sequencing technology
Illumina
Output
> 10 M clusters per sample
Deliverables
Project report & FASTQ files
SRS Flex
Species
Mammalian; others upon request
RNA amount
> 1 ng
RNA quality
Variable RNA quality
Sequencing technology
Illumina
Output
Flexible
Deliverables
Project report & FASTQ files
SRS: Small RNA Sequencing
Bioinformatics
Raw sequencing data are automatically processed in order to facilitate immediate genetic evaluation. Included in delivery are demultiplexed and adapter trimmed FASTQ files.
Further options for bioinformatic analyses are available for human samples:
- Alignment of trimmed sequencing data (BAM format)
- Counting of miRNAs
- Normalization with miRge
- Annotation with miRBase
- Differential gene expression (reads can be mapped to known miRNAs and pre-miRNAs; minimum three replicates per group)
Technical Information
Small RNA sequencing is performed using state-of-the-art Illumina NovaSeq 6000 Sequencing Systems, single end (1x 50 bp) with at least 10 M reads per sample.
If you require sequencing parameters other than those presented in our product portfolio, please let us know! We can provide further solutions.
Contact Us
Start Your Project Now
We are happy to discuss sequencing options and to find a solution specifically tailored to your clinical study or research project.
When getting in contact, please specify sample information including starting material, number of samples, preferred library prep option, preferred sequencing depth and required bioinformatics, if possible.
Further Reading
