Coding transcriptome Sequencing (CTS Classic)
CeGaT offers a highly reproducible protocols for a wide range of species for coding transcriptome sequencing. Using our specific mRNA library preparation kit, we offer a consistent workflow with a minimal input of total RNA as starting material (Figure 4a). Since mRNA is targeted via its poly-A tail, it is suggested to use good quality RNA with a RIN (RNA Integrity Number) > 8 to ensure unbiased sequencing data. We offer mRNA sequencing for eukaryotes with a recommended output of 30 M clusters per sample using PE100 sequencing.
Whole transcriptome sequencing (WTS Classic, WTS Deep, TS Flex)
Our total RNA sequencing service provides a reliable and reproducible workflow for analyzing mRNA, as well as many types of non-coding RNA and with the option to deplete ribosomal RNA (Figure 4b). Even challenging samples with, e.g., moderate or slightly fragmented RNA, perform well with our whole transcriptome sequencing protocol. Total RNA of various quality can be used to produce a usable sequencing library. We offer whole transcriptome sequencing for e.g. human, mouse or rat samples. Furthermore, we established workflows to process total RNA samples isolated from human blood, tumors, plants and bacteria. Depending on the sample, RIN values lower than 8 can be accepted. We recommend a sequencing output of 30 M or 50 M clusters, but customization is possible. For more information please get in touch with us.